Thanks in advance for any guidance, ideas, and/or discussion. RTC Data Stewardship (RTC DS) manages the Radboudumc GraphPad Prism license and it came their attention, that several departments are not. My question is: How do I take the ‘intrinsic activity’ of the enzyme into account (if I need to at all)? Can anyone recommend an equation and appropriate software that can do this? We currently have SigmaPlot, GraphPad, and Enzfitter (for the double substrate inhibition model).

We’ve run a full course of analyses, varying the NADH as well as the substrate and we're now trying to fit the data to an appropriate equation.

The intrinsic activity introduces a baseline velocity and is complicating our kinetic analyses a bit. Complicating it even further is a noted double substrate inhibition pattern (apparently not uncommon with ping-pong mechanism enzymes). Of course, upon addition of substrate, the velocity of the reaction increases and NADH is consumed more rapidly.

Please contact us if you have any questions or would like to renew your license/s.įor all Software Licensing questions please go to ITS Service Desk.I’m working with a rather complicated ping pong, bi-bi double displacement reductase enzyme that oxidizes NADH and then reduces a substrate. Interestingly, the enzyme has intrinsic NADH oxidase activity in the absence of the substrate. UCSD Prism agreement renewal starts the first week of February 2023. We'll quote the price once we receive your software form request. Full name and UCSD email address of user/s who need a license 2. Please submit a Software Form request and provide the following information to recharge the account. UCSD Prism software agreement is renewed for another year and Prism licenses are good for the period of. UCSD Researchers can get access to Prism software. You can organize your data effectively, perform the right analysis, get actionable help as you go, graph, and share your work. Prism is the preferred analysis and graphing solution purpose-built for scientific research. Available to: UCSD Researchers Platforms: Windows & Mac

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